The real-time fluorescence quantitative PCR instrument consists of a fluorescence quantitative system and a CCD system. Through the real-time monitoring of the CCD, the fluorescence during the cycle is monitored. A computer connected to the real-time device collects fluorescence data. The data is displayed in the form of graphs through the developed real-time analysis software. Raw data were plotted as fluorescence intensity versus cycle number. Analysis can begin after raw data has been collected. The real-time device’s software can normalize the collected data to compensate for differences in background fluorescence. After normalization, threshold levels can be set and fluorescence data can be analyzed.

Real-time fluorescence quantitative PCR instrument light is widely used in industries and fields such as basic medical research, pathogen detection, molecular cloning, genetic screening, gene expression, genotyping, transgenic detection, food safety testing, and public health epidemic monitoring.